[phenixbb] poor phasing

Paul Adams PDAdams at lbl.gov
Mon Apr 27 08:25:30 PDT 2009


As Tom suggests you may need to try P41212 - your systematic absences  
can't distinguish between this and P43212.

Alternatively download the latest nightly build of Phenix and try that:

http://www.phenix-online.org/download/nightly_builds.cgi

On Apr 27, 2009, at 5:30 AM, vennila Natesan wrote:

> Dear Tom
>
> Thankyou for your kind reply.I checked my data witn phenix.xtriage
> and there are good anomalous signal (>0.05)in all the resolution  
> shells.
> Also the systematic absences show it is P43212.Still i couldnt
> solve using phenix.Where is the thing going wrong?
> Xtriage results also showed no twinning.
>
>
> My previous message:
> I am using phenix1.4-4 version. In autosol, got the score of 10.24  
> for one iodine soaked data.
> FOM:0.27 and only 54 residues ( that too wrongly) were built out of  
> 129.(CC=0.4), R=0.49 and Rfree=0.574.
> Regrading the data quality, Rsym:0.0448, Ranom:0.055, Resln:25.0-2.6A
> Spacegroup is P43212, completeness:99.4%, Multiplicity:11.7, anomalos
> signal is 1.52 at lowest resoln and higher in high resolution shells.
>
> What might be reason for not  able to solve this structure?
>
>
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-- 
Paul Adams
Acting Division Director, Physical Biosciences Division, Lawrence  
Berkeley Lab
Adjunct Professor, Department of Bioengineering, U.C. Berkeley
Vice President for Technology, the Joint BioEnergy Institute
Head, Berkeley Center for Structural Biology

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