[phenixbb] Very poor density, but very important loops

[Waight, Andrew]

Hello All, 

               I am putting the finishing touches on my structure, a novel membrane protein channel. The data extends to 2.1A and the R factors are currently at ~17/21. However there is a certain loop/helical region where the density gets pretty poor, Unfortunately this is a relatively important ligand binding site so I would like to extract as much information from my patchy blobs as possible. I am wondering if generating a kick map would be useful in this scenario. If so am I missing something other than setting use_kick_maps = True and adding a couple more map definition labels (as described by Pavel)? Also does anyone have any advanced technique suggestions for this type of circumstance, i.e. would it make sense to combine multiple reflection datasets? Thanks so much for all your help, I am a true phenix enthusiast and feel like I owe quite a bit to all of you who have made this amazing program possible. 

                                              Drew Waight



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