[phenixbb] How do different sections of TLS groups affect refinment

[Kendall Nettles]

The tlsmd documentation is very helpful in explaining the rationale.

There are many examples of helices flexing or breaking in comparing  
strucures of the same protein bound to different ligands, so don't  
worry about that.

You might find it worthwhile to try a few different tls groups in  
parallel. In my experience, lower resolution structures are more  
sensitive to the number of groups.

Kendall Nettles

On Jan 20, 2010, at 4:40 PM, "Bart, Aaron G" <dharma at ku.edu> wrote:

>
> Greetings everyone,
> I am still fairly new to crystallography, so forgive me for my
> naivety.
>
> My question involves how the TLSMD server segments my protein
> and the effects it has on refinement. For instance when i use
> "Multi-Chain Alignment Analysis", which lines the sequence for
> all of my chains (a total of 12) and shows how TLS groups are
> divided among them, it shows varying selections of TLS groups
> among the different chains. It should be noted that the sequence
> is the same for all the chains in my protein.
>
> Also if a TLS group splits a alpha helix into multiple parts, would
> this be valid? I have come to understand that helices move more like
> rigid bodies than segmented groups...
>
> Thanks!
>
> -Aaron
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