[phenixbb] Post-doctoral positions at the NKI /Amsterdam to work on Autotaxin
Anastassis Perrakis
a.perrakis at nki.nl
Wed Jan 19 02:35:17 PST 2011
Dear all,
Post-doctoral positions are available in the Netherlands Cancer
Institute in the group of Tassos Perrakis (http://xtal.nki.nl).
Our group, together with that of Wouter Moolenaar and of Huib Ovaa,
have a long-standing interest and lively collaboration in the function
and structure of Autotaxin (ATX), a human enzyme that produces the
well-established lipid mediator LPA. ATX has a very promising
potential as a drug target. Together also with our collaborators at
Pfizer and at Kentucky (Andrew Morris) we have recently published the
structure of Autotaxin alone and in complex with our favorite
inhibitor, as well as data that argue for an interaction of ATX with
integrins.
Structure: http://www.nature.com/nsmb/journal/vaop/ncurrent/full/nsmb.1980.html
Crystals (good fun!): http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935246/?tool=pubmed
Our chemistry collaborators (Ovaa group at the NKI) have also recently
published data on potent inhibitors that act in vivo, as well as an
activity-based probe.
Best Inhibitor: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2867685/?tool=pubmed
Inhibitor design: http://pubs.acs.org/doi/abs/10.1021/jm1005012
Activity based probe: http://onlinelibrary.wiley.com/doi/10.1002/cbic.201000349/abstract;jsessionid=D15B330C3E290F27688458F751F7FC11.d03t02
We are looking for highly motivated post-doctoral scientists to work
on projects related to the function of Autotaxin, aiming to understand
how Autotaxin activity regulates the production of LPA and how we can
design more potent inhibitors and activity based probes. The project
includes protein and mutant production, crystallographic studies and/
or cell biology analysis, and is funded by the KWF (Netherlands Cancer
Fund) and the NWO (Netherlands Science Organization).
The Netherlands Cancer Institute (http://www.nki.nl) is a center of
excellence with a high standard of biological research and an
interactive international atmosphere. It is located in Amsterdam, with
all its cultural amenities, close to Schiphol airport. You will join a
lively team, closely associated with that of Titia Sixma in the
Department of Biochemistry and next to the NKI Protein facility.
http://xtal.nki.nl/
http://proteinfacility.nki.nl/
The research teams at B8 are closely associated and have an interest
in structural studies coupled to functional analysis, combined with
activities in method development for structural biology. Our
department also hosts the newly established "Protein Facility" with
equipment that includes high throughput crystallization and automated
crystal visualization robotics both for nano-drop and Fluidigm micro-
fluidics experiments, an X-ray facility (with Micro-star Ultra source
from Bruker and a MarDTB), Biacore surface plasmon resonance,
isothermal titration calorimetry (ITC), static light scattering
(MALLS) , Thermofluor, an automated protein expression testing
platform, a mass-spectrometry unit, as well as facilities to express
protein in E.coli, insect and mammalian cells.
We are looking for enthusiastic researchers, which could have
experience in protein crystallography and/or molecular biology and/or
biochemistry and/or cell biology. Applicants should send an e-mail
including a formal CV, a very short motivation letter for why they
want to work on this project, and include the names of three referees,
and send it to me.
Tassos
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