[phenixbb] Subject: no kidding - we need small crystals!
Filip YABUKARSKI
fyabukar at embl.fr
Thu Jul 5 12:33:19 PDT 2012
Hello,
Definitely try microseeding: take a big crystal, put it in eppendorf
with mother liquor ( the precipitant should be at a concentration higher
than what you use to build the big crystals) and crash it by vortexing
(put a small piece of plastic in the tube). If you use the vapor
diffusion technique take a µl of this solution and mix it with your
protein solution on a cover slip just as if you set a normal drop, and
then seal the well. Normally youll get a lot of tiny crystals after day
or two. Good luck
Filip
On 2012-07-05 01:55, jens j birktoft wrote:
> Hi everybody,
>
> Any suggestions regarding how to generate small!! crystals? Yes, in
> this case that is what we want While we can generate crystals of size
> 100-500 microns, this time we something in the range of .5-5 microns.
> Any ideas and suggestions would be greatly appreciated,
>
> Cheers
>
> Jens
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