[phenixbb] Ligand building/refinement woes - works now

Machius, Mischa Christian mischa_machius at med.unc.edu
Mon Jan 7 11:11:07 PST 2013


Ok, everything works fine when using the latest development version of Phenix.

Before I got an error saying something about a keyword not recognized when dealing with chiral centers in the ligand. I can rerun it to capture the error report, if it's of interest.

Cheers,
MM



On Jan 7, 2013, at 12:27 PM, "Machius, Mischa Christian" <mischa_machius at med.unc.edu>
 wrote:

> Sorry, I mistyped. I am working with NAP (NADP).
> 
> 
> 
> On Jan 7, 2013, at 12:18 PM, "Schubert, Carsten [JRDUS]" <CSCHUBER at its.jnj.com>
> wrote:
> 
>> Hi Mischa,
>> 
>> I use ligand code NDP for NADPH. I can send you PDB and cif files if you like. 
>> 
>> Cheers,
>> 
>> 	Carsten
>> 
>> -----Original Message-----
>> From: phenixbb-bounces at phenix-online.org [mailto:phenixbb-bounces at phenix-online.org] On Behalf Of Machius, Mischa Christian
>> Sent: Monday, January 07, 2013 10:30 AM
>> To: mailing list user PHENIX
>> Subject: [phenixbb] Ligand building/refinement woes
>> 
>> I seem to be having the hardest time building/refining NADPH (PDB code NAP).
>> 
>> When fetching this common ligand from within coot, Phenix (1.81-1168) subsequently does not seem to accept it and bombs noting all kinds of violations.
>> 
>> When I have Phenix automatically build NAP into the density, it does place NAP (albeit completely out of otherwise quite nice density). When I then take the coordinates, read them into coot together with the CIF that Phenix writes into the ligand_build directory, placing and refinement in coot works well, but subsequent refinement in Phenix, using the very same CIF, again bombs. Since hydrogens are often an issue when going from coot to Phenix I remove them before running Refine. I have also tried just refining the coordinates as they came out of ligand_build. But Phenix still bombs and doesn't seem to accept its own CIF.
>> 
>> I would have thought that a ligand as common as NAP wouldn't require a lot of gymnastics, perhaps not even reading CIF information, and that it would go all automatically.That's probably too optimistic on my part.
>> 
>> It's probably something silly, but I can't figure out what it is. 
>> 
>> Any ideas?
>> 
>> Many thanks in advance,
>> MM
>> 
>> 
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