[phenixbb] problem with MR

Nathaniel Echols nechols at lbl.gov
Tue Apr 29 16:17:53 PDT 2014


On Tue, Apr 29, 2014 at 5:34 PM, Subhani Bandara <ramssb17 at gmail.com> wrote:

> I am trying to solve the structure of 7 KDa protein with a cubic space
> group at 1.35A resolution with Phaser-MR. It gives the error massage saying
> "The composition you have entered has a protein/nucleic acid content of
> 300.3%, which is impossible. Decrease your composition" and stops.  This
> appears even if I give one chain as search model and say there is only one
> component copy. How can I fix this.
>

The most likely explanation is that your sequence file contains multiple
copies - or perhaps it contains the entire sequence of a large protein and
you're working with a single domain?

-Nat
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