[phenixbb] scaling in Fo-Fo maps
pafonine at lbl.gov
Thu Feb 4 09:59:19 PST 2016
> In line with the question of Xun, will you please introduce a way to
> scale-up the intensity of cryo-EM to the level of intensity of x-ray
map comparison does not care where maps come from. You give it two maps
on the same gridding (same gridding is essential, obviously) and it
tells you pairs of contouring levels that will show equivalent
representations of your maps.
> I have a read a paper, in which the author have done the
> above-mentioned scaling-up in order to use the phenix reciprocal space
> refine for cryo-EM data refine,
Normally, you should not use reciprocal spare refinement when working
with cryo-EM maps as your data, cryo-EM map, is in real space. And if
you use real-space refinement it does not matter on what scale the input
map is as it rescales it internally anyway.
> Currently with the availability of pheinix.real_space_refine, in a lot
> of situations reciprocal refine for cryo-EM data is still necessary.
I would be interesting to hear about those cases!
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