[phenixbb] FSC curve between experimental cryo EM map and model
pafonine at lbl.gov
Fri Apr 28 18:10:14 PDT 2017
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On 4/28/17 06:08, #AHMED TOFAYEL# wrote:
> Dear all,
> If I want to calculate FSC curve between experimental cryo EM map and
> model, what is the correct procedure to do so?
> I will explain what I have tried so far. Although I have variable
> local resolution in my map, according to FSC=0.143 criterion I have a
> 4 Ang map (say 1.mrc), I have used 'molmap' command in Chimera to
> generate a simulated map at 4 Ang. Then I used "vop resample" to take
> care of the pixel and box values ( then saved as 2.mrc). Thereafter, I
> have used "e2proc3d.py 1.mrc fsc.txt --calcfsc=2.mrc and plotted the
> fsc.txt file to get the curve.
> I am not sure if I am on the right track. My pixel size is 1.28.
> Best regards,
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