[phenixbb] Phenix Phaser Question

[Randy John Read]

Dear Hyun Jeong,

It’s worrying if you get highly ambiguous solutions in Phaser, because most of the time a correct solution is unique. 

Could you email me the log file from the job that found 18 possible solutions (preferably off-list)? That might give some ideas about what is going on.

Best wishes,

Randy Read

> On 11 Jul 2023, at 05:29, hyun4867 <hyun4867 at korea.ac.kr> wrote:
> 
> Dear, Everyone
> 
> Hi all, I am Hyun Jeong.
> I want to ask you about phenix phaser problem.
> 
> I obtain diffraction pattern of crystal that is complex.
> I tried to solve the phase based on the Apo structure i previoulsy solved.
> 
> Using full-featured phaser, i obtain 18 Phaser solution. This is really strange number.
> But, the map obtained 18 Phaser solution is fitted in electron density map.
> 
> May I proceed with processing using the discovered electron map with 18 Phasers?
> Is it necessary to redo the scaling?
> 
> I appreciate your request for advice.
> 
> Best regards,
> Hyun Jeong
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-----
Randy J. Read
Department of Haematology, University of Cambridge
Cambridge Institute for Medical Research     Tel: +44 1223 336500
The Keith Peters Building
Hills Road                                                       E-mail: rjr27 at cam.ac.uk
Cambridge CB2 0XY, U.K.                              www-structmed.cimr.cam.ac.uk