<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">I am refining a structure containing SeMet with data collected at peak wavelength. Provided I give the f' and f'', will phenix.refine calculate F+ and F- structure factors and use the provided FOBS+ and FOBS- ? CNS does this. Refmac only uses f' and I get >6 sigma Fo-Fc peaks on the Se.<div><br class="webkit-block-placeholder"></div><div>Thanks for your help.<div><div><div> <p style="margin: 0.0px 0.0px 0.0px 0.0px"><font face="Helvetica" size="3" style="font: 12.0px Helvetica">_________________________________</font></p> <p style="margin: 0.0px 0.0px 0.0px 0.0px"><font face="Helvetica" size="3" color="#004080" style="font: 12.0px Helvetica; color: #004080"><b>Mark A. Saper, Ph.D.</b></font></p> <p style="margin: 0.0px 0.0px 0.0px 0.0px"><font face="Helvetica" size="3" style="font: 12.0px Helvetica">Associate Professor of Biological Chemistry</font></p> <p style="margin: 0.0px 0.0px 0.0px 0.0px"><font face="Helvetica" size="3" style="font: 12.0px Helvetica">Biophysics Research Division, University of Michigan</font></p> <p style="margin: 0.0px 0.0px 0.0px 0.0px"><font face="Helvetica" size="3" style="font: 12.0px Helvetica">930 N University Ave</font></p> <p style="margin: 0.0px 0.0px 0.0px 0.0px"><font face="Helvetica" size="3" style="font: 12.0px Helvetica">Ann Arbor MI 48109-1055 <span class="Apple-converted-space"> </span>U.S.A.</font></p> <p style="margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica; min-height: 14.0px"><br></p> <p style="margin: 0.0px 0.0px 0.0px 0.0px"><font face="Helvetica" size="3" style="font: 12.0px Helvetica"><a href="mailto:saper@umich.edu">saper@umich.edu</a><span class="Apple-converted-space"><span class="Apple-converted-tab"> </span></span>(734) 764-3353<span class="Apple-converted-space"><span class="Apple-converted-tab"> </span></span>fax (734) 764-3323</font></p> <p style="margin: 0.0px 0.0px 0.0px 0.0px"><font face="Helvetica" size="3" style="font: 12.0px Helvetica"><a href="http://www.biochem.med.umich.edu/biochem/research/profiles/saper.html">http://www.biochem.med.umich.edu/biochem/research/profiles/saper.html</a></font></p> </div><br></div></div></div></body></html>