Dear all<br>I was trying to refining a low resolution structure with Phenix.<br>The crystal belongs to the SG P6122 with one monomer per ASU. The monomer contains <br>approximately 270 residues. There isn't any NCS operators. <br>
The crystal diffracts up to 4.0 Angstrons, with good statistics, however I have only 4204 unique reflections.<br>I solved the structure by MR by Phaser and the solution was quite reasonably. <br>Initially I performed a phenix refining cicle with SA + rigid body, however the Rs values is very divergent (43 and 59%),<br>
so I decided to use the key xray_data.r_free_flags.ignore_r_free_flags=true in order to save my precious reflections with and without <br>SA, and I also use the group_adp., in those cases the R is about 39-40%. <br>I want a help to decide the best refinement strategies for low resolution refinement with phenix.<br>
BTW I'm trying to produce some different constructions of the gene, expecting improve the resolution. <br><br>Thanks<br>Humberto<br><br><br><br>