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<TITLE>How to generate an anomalous map in PHENIX?</TITLE>
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<P><FONT FACE="Arial">Hi,</FONT>
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<P><FONT FACE="Arial">I am trying to make an anomalous map in the latest phenix. After refinement of a medium resolution structure (1.8A) with anomalous data (and signal) I used the ANOM and PHANOM columns in the map_coeffs.mtz file to generate a map. The map was created either in coot or via fft, but shows no anomalous peaks for a present Cl1 nor for SS bonds. It rather mimics a noisy protein map. Generation of an anomalous map in CNX using the same data (FOBS(+/-) and HL coeffs(calc) ) produces a nice map with the expected peaks. Am I misunderstanding the purpose of the ANOM/PHANOM columns or is this feature not implemented yet.</FONT></P>
<P><FONT FACE="Arial">Cheers</FONT>
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<P> <FONT FACE="Arial">Carsten</FONT>
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