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Dear all --<div><br><div><div>On 27 Aug 2008, at 04:37, Pavel Afonine wrote:</div><blockquote type="cite"><p style="margin: 0.0px 0.0px 0.0px 0.0px"><font face="Helvetica" size="3" style="font: 12.0px Helvetica">I'm not sure I understand why you don't want to use your original file<span class="Apple-converted-space"> </span></font></p> <p style="margin: 0.0px 0.0px 0.0px 0.0px"><font face="Helvetica" size="3" style="font: 12.0px Helvetica">with reflections...</font></p> </blockquote><br></div><div>As a fact, I have a question to the all community... why would people refine against reflections different that the "true" ones, e.g. the ones obtained directly after indexing? My thought was that if one tries to refine against, let say, modified reflections, it will result with model bias, and "wrong" interpretation of the data. At least, this is what I'm telling my students: ALWAYS refine against your ORIGINAL reflections!</div><div><br></div><div>Am I missing something there?</div><br><div> <span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div>Kind regards.</div><div><br class="khtml-block-placeholder"></div><div>-- Leo --</div><div>------------------------------------------------------------</div><div>Chavas Leonard, Ph.D. @ Osaka, Japan</div><div>Research Associate</div><div>Marie Curie Actions Fellow</div><div>------------------------------------------------------------</div><div>Faculty of Life Sciences</div><div>The University of Manchester</div><div>The Michael Smith Building</div><div>Oxford Road</div><div>Manchester Lancashire</div><div>M13 9PT</div><div>------------------------------------------------------------</div><div>Tel: +44(0)161-275-1586</div><div>e-mail: <a href="mailto:Leonard.Chavas@manchester.ac.uk">Leonard.Chavas@manchester.ac.uk</a></div><a href="http://personalpages.manchester.ac.uk/staff/leonard.chavas/">http://personalpages.manchester.ac.uk/staff/leonard.chavas/</a><br class="Apple-interchange-newline"><br class="Apple-interchange-newline"></span></span></span> </div><br></div></body></html>