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<TITLE>AutoBuild can't align sequence file</TITLE>
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<P><FONT SIZE=2>Hi all, i'm currently trying to solve my structure with phenix and i tried to do it via AutoMR. At first everything works fine, it finds a MR solution, but then i get this error:<BR>
<BR>
Chain too short or poor match to sequence: MYIQVLGSAAGGGFPQWNCNCVNCKGYRDGTLKATARTQSSIALSDDGVHWILCNASPDIRAQLQAFAPMQPARALRDTGINAIVLLDSQIDHTTGLLSLREGCPHQVWCTDMVHQDLTTGFPLFNMLSHWNGGLQWNRIELEGSFVIDACPNLKFTPFPLRSAAPPYSPHRFDPHPGDNLGLMVEDTRTGGKLFYAPGLGQVDEKLLAMMHGADCLLVDGTLWEDDEMQRRGVGTRTGREMGHLAQNGPGGTLEVLDGFPRQRKVLIHINNTNPILDENSPERAEVLRRGVEVAFDGMSIELL<BR>
<BR>
NOTE: chain A (residues 1-304) MYIQVLGSAAGGGFPQWNCNCVNCKGYRDGTLKATARTQSSIALSDDGVHWILCNASPDIRAQLQAFAPMQPARALRDTGINAIVLLDSQIDHTTGLLSLREGCPHQVWCTDMVHQDLTTGFPLFNMLSHWNGGLQWNRIELEGSFVIDACPNLKFTPFPLRSAAPPYSPHRFDPHPGDNLGLMVEDTRTGGKLFYAPGLGQVDEKLLAMMHGADCLLVDGTLWEDDEMQRRGVGTRTGREMGHLAQNGPGGTLEVLDGFPRQRKVLIHINNTNPILDENSPERAEVLRRGVEVAFDGMSIELL<BR>
did not match the input sequence...<BR>
ignoring alignment and rebuilding segment without sequence information.<BR>
***************************************<BR>
AutoBuild Input failed<BR>
<BR>
Sorry, the PDB file and sequence file could not be aligned<BR>
(with no gaps and >50.0% identity)<BR>
<BR>
Please restart the wizard...<BR>
You have several possibilities to try ....<BR>
You can set rebuild_in_place=No<BR>
You can set input_sequence_file=None<BR>
You can set min_seq_identity_percent to a lower value<BR>
You can set highest_resno to a higher value<BR>
You can specify start_chains_list if your <BR>
PDB file sequence starts with a residue number greater than the<BR>
number of residues in the sequence.<BR>
<BR>
<BR>
what can i do now? the pdb file has nearly the same sequence (2 mutations) but he says that he can't align them, i use a .dat file for this is this correct? i also have a fasta line in it >... but it doesn't work without this line either.<BR>
i tried with different phenix versions and have always the same problem<BR>
Any suggestions would be appreciated.<BR>
<BR>
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