<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Hi PC,<div><br></div><div>I'm sorry for the trouble! I'm not quite clear at what point the error message came up, but I would recommend downloading a current version of PHENIX from the web site (go to the "nightly builds" list on the download site and get a recent one) because that error should be fixed!</div><div><br></div><div>Once you complete phenix.autosol, then you should be able to run autobuild with the results from autosol and it will do what you want, combining the model with the experimental phases to yield an improved map and model.</div><div><br></div><div>All the best,</div><div>Tom T</div><div><br><div><div>On Jul 12, 2009, at 11:19 AM, Pei-Chun Lin wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0; "><div class="hmmessage" style="font-size: 10pt; font-family: Verdana; ">Hi,<span class="Apple-converted-space"> </span><br><br>I am the beginner of crystallography and have a 3.5A map generated by MAD method using phenix GUI.<span class="Apple-converted-space"> </span><br>I already traced and built 2/3 of my protein.<br>I wish to use this partial model combined with SAD to generate a new map and see if the new map can help me to deal with the rest of the structure.<br>Does it sound reasonable to you?<br><br>However, after running AutoSol. I got this error message:<br><br>Failed to carry out AutoSol_score_ha_solutions:<br>[Errno 2] No such file or directory: 'AutoSol_run_1_/TEMP0/phaser_mtz.hand.mtz'<br><br>What should I do now? Can't phenix automatically calculate it?<span class="Apple-converted-space"> </span><br><br><br>PC<span class="Apple-converted-space"> </span><br><br><hr>Windows Live�: Keep your life in sync.<span class="Apple-converted-space"> </span><a href="http://windowslive.com/explore?ocid=TXT_TAGLM_WL_t1_allup_explore_012009" target="_new">Check it out!</a><span class="Apple-converted-space"> </span>_______________________________________________<br>phenixbb mailing list<br><a href="mailto:phenixbb@phenix-online.org">phenixbb@phenix-online.org</a><br><a href="http://www.phenix-online.org/mailman/listinfo/phenixbb">http://www.phenix-online.org/mailman/listinfo/phenixbb</a><br></div></span></blockquote></div><br><div apple-content-edited="true"> <span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div><div><div><div><div><br>Thomas C. Terwilliger<br>Mail Stop M888<br>Los Alamos National Laboratory<br>Los Alamos, NM 87545<br><br>Tel: 505-667-0072 email: <a href="mailto:terwilliger@LANL.gov">terwilliger@LANL.gov</a><br>Fax: 505-665-3024 SOLVE web site: <a href="http://solve.lanl.gov">http://solve.lanl.gov</a><br>PHENIX web site: http:<a href="http://www.phenix-online.org">www.phenix-online.org</a><br>ISFI Integrated Center for Structure and Function Innovation web site: <a href="http://techcenter.mbi.ucla.edu">http://techcenter.mbi.ucla.edu</a><br>TB Structural Genomics Consortium web site: <a href="http://www.doe-mbi.ucla.edu/TB">http://www.doe-mbi.ucla.edu/TB</a></div><div>CBSS Center for Bio-Security Science web site: <a href="http://www.lanl.gov/cbss">http://www.lanl.gov/cbss</a><br><br></div></div></div></div><br></div></div></span><br class="Apple-interchange-newline"> </div><br></div></body></html>