Hi Pavel,<br><br>Thank you very much for your fast answer. I just thought that maybe the bulk solvent correction procedure in phenix can really create better maps because with such big complexes I must use all low resolution data. Of course I can try both options but there are so many things to try with this big complex at low resolution that I would like to eliminate options where possible. <br>
<br>Many many thanks,<br><br>Peter.<br><br><br><br><div class="gmail_quote">On Sun, Aug 30, 2009 at 7:22 PM, Pavel Afonine <span dir="ltr"><<a href="mailto:PAfonine@lbl.gov">PAfonine@lbl.gov</a>></span> wrote:<br><blockquote class="gmail_quote" style="border-left: 1px solid rgb(204, 204, 204); margin: 0pt 0pt 0pt 0.8ex; padding-left: 1ex;">
Hi Peter,<br>
<br>
I guess this my reply will not replace Tom's or Randy's one...<br>
<div class="im"><br>
> Should it be the 2mFo-DFc map directly from Phaser or would it be<br>
> better to do rigid body refinement in phenix.refine and use the bulk<br>
> solvent corrected 2mFo-DFc map ?<br>
<br>
</div>- why don't you try using both options and choose the best outcome?<br>
<br>
- running a round of rigid body refinement in phenix.refine and using<br>
its 2mFo-DFc map will not hurt at least or may make your model and<br>
therefore the map better, so you will have a better starting point.<br>
<br>
Pavel.<br>
<br>
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</blockquote></div><br>