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PS: Make sure you use not fobs-filled map (xxx_no_fill).<br>
<br>
Pavel.<br>
<br>
<br>
On 8/30/09 2:32 PM, Peter Grey wrote:
<blockquote
cite="mid:4a26094b0908301432o271823a2s763da769b4a01bbd@mail.gmail.com"
type="cite">Hi Pavel,<br>
<br>
Thank you very much for your fast answer. I just thought that maybe the
bulk solvent correction procedure in phenix can really create better
maps because with such big complexes I must use all low resolution
data. Of course I can try both options but there are so many things to
try with this big complex at low resolution that I would like to
eliminate options where possible. <br>
<br>
Many many thanks,<br>
<br>
Peter.<br>
<br>
<br>
<br>
<div class="gmail_quote">On Sun, Aug 30, 2009 at 7:22 PM, Pavel
Afonine <span dir="ltr"><<a moz-do-not-send="true"
href="mailto:PAfonine@lbl.gov">PAfonine@lbl.gov</a>></span> wrote:<br>
<blockquote class="gmail_quote"
style="border-left: 1px solid rgb(204, 204, 204); margin: 0pt 0pt 0pt 0.8ex; padding-left: 1ex;">Hi
Peter,<br>
<br>
I guess this my reply will not replace Tom's or Randy's one...<br>
<div class="im"><br>
> Should it be the 2mFo-DFc map directly from Phaser or would it be<br>
> better to do rigid body refinement in phenix.refine and use the
bulk<br>
> solvent corrected 2mFo-DFc map ?<br>
<br>
</div>
- why don't you try using both options and choose the best outcome?<br>
<br>
- running a round of rigid body refinement in phenix.refine and using<br>
its 2mFo-DFc map will not hurt at least or may make your model and<br>
therefore the map better, so you will have a better starting point.<br>
<br>
Pavel.<br>
<br>
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