<html><head><style type="text/css"><!-- DIV {margin:0px;} --></style></head><body><div style="font-family:times new roman,new york,times,serif;font-size:12pt"><div>Dear Tom<br><br>thanks.<br></div><div style="font-family: times new roman,new york,times,serif; font-size: 12pt;"><br><div style="font-family: times new roman,new york,times,serif; font-size: 12pt;"><font face="Tahoma" size="2"><hr size="1"><b><span style="font-weight: bold;">From:</span></b> Tom Terwilliger <terwilliger@lanl.gov><br><b><span style="font-weight: bold;">To:</span></b> PHENIX user mailing list <phenixbb@phenix-online.org><br><b><span style="font-weight: bold;">Sent:</span></b> Wednesday, September 2, 2009 9:20:55 AM<br><b><span style="font-weight: bold;">Subject:</span></b> Re: [phenixbb] low resolution map<br></font><br>
Hi Venkat,<div>Yes, you can use autobuild to create a map including model and experimental phase information:</div><div><br></div><div>phenix.autobuild data=sad_phase_info.mtz model=partial_mr_solution.pdb \</div><div>seq_file=seq.dat maps_only=true </div><div><br></div><div>This will refine the partial model, use it along with the sad phases in density modification, and produce coefficients for a new map for you:</div><div><br></div><div><div>--- Density-modified map coefficients FP PHIM FOM ---</div><div>hklout_denmod: AutoBuild_run_3_/cycle_best_2.mtz</div><div><br></div><div><br></div></div><div>All the best,</div><div>Tom T</div><div><br></div><div><br><div><div>On Sep 2, 2009, at 8:12 AM, r n wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant: normal;
font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px;"><div><div style="margin: 0px; font-family: 'times new roman','new york',times,serif; font-size: 12pt;"><div style="margin: 0px;">Hi<span class="Apple-converted-space"> </span><br><br>I would like to know for low resolution data with poor phase information (SAD and partial MR), Is there possibility to<br>in Phenix to improve the phase and map using the partial model at low resolution (3.5 Ang), similar to TNT/BUSTER.<br><br>Thanks<br>venkat<br></div><div style="margin: 0px; font-family: 'times new roman','new york',times,serif; font-size: 12pt;"><br><div style="margin: 0px; font-family: arial,helvetica,sans-serif; font-size: 13px;"><font face="Tahoma" size="2"><hr size="1"><b><span style="font-weight:
bold;"></span></b></font><br></div></div></div><br>_______________________________________________<br>phenixbb mailing list<br><a rel="nofollow" ymailto="mailto:phenixbb@phenix-online.org" target="_blank" href="mailto:phenixbb@phenix-online.org">phenixbb@phenix-online.org</a><br><span><a target="_blank" href="http://www.phenix-online.org/mailman/listinfo/phenixbb">http://www.phenix-online.org/mailman/listinfo/phenixbb</a></span><br></div></span></blockquote></div><br><div> <span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px;"><div style="word-wrap: break-word;"><div><div><div><div><div><br>Thomas C. Terwilliger<br>Mail Stop M888<br>Los Alamos National Laboratory<br>Los
Alamos, NM 87545<br><br>Tel: 505-667-0072 email: <a rel="nofollow" ymailto="mailto:terwilliger@LANL.gov" target="_blank" href="mailto:terwilliger@LANL.gov">terwilliger@LANL.gov</a><br><span>Fax: 505-665-3024 SOLVE web site: <a target="_blank" href="http://solve.lanl.gov">http://solve.lanl.gov</a></span><br>PHENIX web site: http:<a rel="nofollow" target="_blank" href="http://www.phenix-online.org">www.phenix-online.org</a><br><span>ISFI Integrated Center for Structure and Function Innovation web site: <a target="_blank" href="http://techcenter.mbi.ucla.edu">http://techcenter.mbi.ucla.edu</a></span><br><span>TB Structural Genomics Consortium web site: <a target="_blank"
href="http://www.doe-mbi.ucla.edu/TB">http://www.doe-mbi.ucla.edu/TB</a></span></div><div><span>CBSS Center for Bio-Security Science web site: <a target="_blank" href="http://www.lanl.gov/cbss">http://www.lanl.gov/cbss</a></span><br><br></div></div></div></div><br></div></div></span><br class="Apple-interchange-newline"> </div><br></div></div></div></div><br>
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