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Hi,<br>
<br>
I hope Tom gives you the answer about how to stop water picking in
autobuild. <br>
<br>
>From my side, I'm wondering why it bothers you (I'm not saying that not
working option is good)? Why don't you consider those waters as a tool
for improving your map? You are free to remove any of them them later
on. This can be viewed as a prototype of what ARP/wARP does, or other
"density growing" techniques... In your case you just temporarily place
waters where the model is still absent, and when you build those
missing places just consider waters as density peaks (I hope model
building programs assume waters as just density peaks and remove waters
if they are on the macromolecule's way). On the other hand, model
incompleteness may make the map ugly even for relatively well defined
places.<br>
<br>
Pavel.<br>
<br>
<br>
On 9/6/09 7:38 AM, crystallogrphy wrote:
<blockquote
cite="mid:33fd30c80909060738p5a4348c8n4c09d51925ee5bb1@mail.gmail.com"
type="cite">Hi,<br>
I am building a model against a 2.1A data set using phenix.autobuild.
Right now Rfree is about 35%. I do not want to pick water in this stage
because I see denisty at N-terminal of my model which need to build
more residues. However, the program still picks water even after I say
'place_waters=False'. Does anyone know why?<br>
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face="Nimbus Roman No9 L, serif">phenix.autobuild
data=exptl_fobs_phases_freeR_flags.mtz
model=AutoBuild_run_4_/overall_best-090905a.pdb
seq_file=../ztb-sd.seq refinement.place_waters=False
refinement.refine_with_ncs=True nproc=2</font></font></p>
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