<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Hi Sacha,<div>Yes, that would help. </div><div><br></div><div>In parallel, to get a little more data on the practical aspects of all this, I think I will do a test to see how much difference taking out a super-test-set of reflections completely makes in overall structure determination from start to finish using fully automated structure determination with phenix.autosol and phenix.autobuild.</div><div><br></div><div>All the best,</div><div>Tom T</div><div><br><div><div>On Oct 1, 2009, at 1:56 AM, Alexandre OURJOUMTSEV wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite">Hi, Tom, Pavel and Joe,<br><br>> I'm glad you put that option in, Pavel. However for model-<br>> building it is not so straightforward. Normally we are building into density-modified<br>> maps. Density modification works poorly when a significant set of<br>> reflections is excluded, so this becomes impractical. <br><br>As I remember it was a paper (by Blanc, ..., Bricogne ? am I wrong? Ifailed to find it right now) where they faced exactly this problem.Again, as I remember they suggested to exclude the test-set reflectionsfrom the map calculation but , to minimize the map deformation, insteadof them include average Fobs for the corresponding resolution shell.<br><br>Is it a way to answer the original question / problem ?<br><br>As I understand, a similar technique Pavel already uses to calculate the maps with Fobs missed.<br><br>Best regards,<br><br>Sacha</blockquote></div><br><div apple-content-edited="true"> <span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div><div><div><div><div><br>Thomas C. Terwilliger<br>Mail Stop M888<br>Los Alamos National Laboratory<br>Los Alamos, NM 87545<br><br>Tel: 505-667-0072 email: <a href="mailto:terwilliger@LANL.gov">terwilliger@LANL.gov</a><br>Fax: 505-665-3024 SOLVE web site: <a href="http://solve.lanl.gov">http://solve.lanl.gov</a><br>PHENIX web site: http:<a href="http://www.phenix-online.org">www.phenix-online.org</a><br>ISFI Integrated Center for Structure and Function Innovation web site: <a href="http://techcenter.mbi.ucla.edu">http://techcenter.mbi.ucla.edu</a><br>TB Structural Genomics Consortium web site: <a href="http://www.doe-mbi.ucla.edu/TB">http://www.doe-mbi.ucla.edu/TB</a></div><div>CBSS Center for Bio-Security Science web site: <a href="http://www.lanl.gov/cbss">http://www.lanl.gov/cbss</a><br><br></div></div></div></div><br></div></div></span><br class="Apple-interchange-newline"> </div><br></div></body></html>