<html><head><style type="text/css"><!-- DIV {margin:0px;} --></style></head><body><div style="font-family:arial,helvetica,sans-serif;font-size:12pt"><div>Hi <br><br>I am trying to refine the model in low resolution and there are multimer in the asu.<br>I did rigid body refinement after MR , molecule did not move much and R/Rfree stays 45 % and If I do<br>the overlap between the monomers, rms is 0.06. <br><br>If I do simulated annealing or any type of refinement (group_adp), Rfree is high and R goes down, but<br>the if do the superposition of the monomer, rms is 1.5 to 1.8. <br><br>So like to know what kind of refinement is can be done in very low resolution between 5 to 6 ang data?<br><br>Is it any strict ncs could be possible in phenix that should not move the monomer too much?<br><br>suggestions are appreciated<br><br>rn<br></div>
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