<html><head><style type="text/css"><!-- DIV {margin:0px;} --></style></head><body><div style="font-family:arial,helvetica,sans-serif;font-size:12pt"><div>Hi <br>I am doing refinement using phenix.<br>I did simulated annealing, Rfree goes up to 10% more than before. (40%) and R stays at 35%.<br>If use previous phenix refinment file for same pdb and data , R and Rfree is 25/27%. <br>I thought even different r-free set after simulated annealing and 7 cycles of refinement in phenix<br>should bring Rfree down, but not ?<br><br>But same data set and model , in CNS, R/Rfree 26/28%.<br><br>Any suggestions will be appreciated.<br><br>I am using phenix-dev249 - linux.<br><br>thanks<br>ram<br></div>
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