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<DIV>Dear all,</DIV>
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<DIV>I have a 2.3A data set processed with HKL2000. The space group is P3121
with 63% of solvent content and 1 molecule in the ASU. I used a 40% homology
model to do molecular replacement. Both the Phaser and Molrep gave similar
solution with good packing. After further refinement with phenix, the built
model fit well with the 2mFo-DFc map. However the R/Rfree were stuck to
0.34/0.38. </DIV>
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<DIV>I rechedked the data set with xtriage and found the data set is merohedral
twinned (with twin law"-h,-k,l", the estimated twin fraction is about 0.05).
When I used the twin law in further refinement, the R/Rfree were only decreased
to 0.32/0.36. Any suggestions are appreciated, thanks!</DIV>
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<DIV>J.X. QI</DIV></BODY></HTML>