<html><head></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div>John,</div><div><br></div><div>I am having the exact same problem. I need the NCS parameters in a format suitable for AutoBuild which I think is different than phenix.refine. Are you trying to use phenix.simple_ncs_from_pdb routine in GUI? Let me know if you have made any progress.</div><div><br></div><div>Thanks,</div><div>Mark</div><div><br></div><blockquote type="cite"><span class="Apple-style-span" style="font-family: Times; "><pre>Hello,
I am having difficulties defining NCS parameters using the GUI
interface (Mac 10.4.11; phenix 1.6-289).
Phenix will automatically select NCS restraint groups and use them
during refinement. But if I edit the automated selections to my
liking then click on Update and Save, the refinement runs with the
original parameters unedited. I have tried reading in a ncs.param
file, but it does not get read during the refinement run. Also, an
intermittent problem with this GUI is that I am not allowed to "Add"
a new restraint group. When I click this button, it just clears out
the selections on the last group, and does not add a new group. Any
ideas?
Thanks.
Best,
John
--
John Pascal, PhD ph 215.503.4596
Assistant Professor fx 215.923.2117
Thomas Jefferson University
Biochemistry & Molecular Biology
233 South 10th Street, BLSB 804
Philadelphia, Pennsylvania 19107</pre></span></blockquote><div><br></div><div>
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">_________________________________</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Helvetica" size="3" style="font: normal normal normal 12px/normal Helvetica; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Helvetica" size="3" style="font: normal normal normal 12px/normal Helvetica; "><span class="Apple-style-span" style="color: rgb(0, 64, 128); font-weight: bold; "><font class="Apple-style-span" color="#BB623F"><font class="Apple-style-span" size="3"><span class="Apple-style-span" style="font-size: 13px; ">Mark A. Saper, Ph.D.</span></font></font><span class="Apple-tab-span" style="white-space: pre; "> </span></span></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font class="Apple-style-span" color="#000000">Associate Professor of Biological Chemistry</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font class="Apple-style-span" color="#000000">University of Michigan</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font style="font: normal normal normal 12px/normal Helvetica; "><font class="Apple-style-span" color="#000000">Biophysics, 3040 Chemistry Building</font></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font style="font: normal normal normal 12px/normal Helvetica; "><font class="Apple-style-span" color="#000000">930 N University Ave</font></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font style="font: normal normal normal 12px/normal Helvetica; "><font class="Apple-style-span" color="#000000">Ann Arbor MI 48109-1055 U.S.A.</font></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><span class="Apple-style-span" style="font-size: medium; "><div apple-content-edited="true"><a href="mailto:saper@umich.edu">saper@umich.edu</a> phone (734) 764-3353 fax (734) 764-3323</div><div apple-content-edited="true"><span class="Apple-tab-span" style="white-space: pre; "><a href="http://www.biochem.med.umich.edu/?q=saper">http://www.biochem.med.umich.edu/?q=saper</a> </span><a href="http://www.strucbio.umich.edu/">http://www.strucbio.umich.edu/</a></div><div><br></div></span></div></span></font></div></span></span></font></span></div></span></div></div></span></span></span></span></span></span></span></span></span></span></div></span></span></span></span></div></span></div></span></div></span></div></span></span><br class="Apple-interchange-newline">
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