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    Hi TriNgo,<br>
    <br>
    once you are at the refinement stage, you can do f',f'' refinement:<br>
    <br>
    <a class="moz-txt-link-freetext" href="http://www.phenix-online.org/documentation/refinement.htm#anch22">http://www.phenix-online.org/documentation/refinement.htm#anch22</a><br>
    <br>
    Pavel.<br>
    <br>
    <br>
    On 9/16/10 11:00 AM, Tom Terwilliger wrote:
    <blockquote cite="mid:8767A6F4-9392-4F78-9ABF-38B441E23BC6@lanl.gov"
      type="cite">Hi TriNgo
      <div><br>
      </div>
      <div>I would suggest (1) see if you can ask the staff at the
        synchrotron about the f' and f" values for Br at the wavelengths
        you used, then if that fails, then (2) just enter in the AutoSol
        GUI the wavelengths and Br and hit "guess f' and f" values".
        &nbsp;This will look up values from a table of scattering factors.
        The values will be refined for you by AutoSol. If you have a
        strong anomalous signal then even if they are off this will work
        fine. However if the signal is weak then having the actual
        values can be helpful.</div>
      <div><br>
      </div>
      <div>All the best,</div>
      <div>Tom T</div>
      <div><br>
        <div>
          <div>On Sep 16, 2010, at 11:55 AM, Tri Ngo wrote:</div>
          <br class="Apple-interchange-newline">
          <blockquote type="cite">Dear Phenix-BB members,
            <div><br>
              <div>I am trying to solve the MAD data of one bacteria
                protein. The crystal was soaked with 1M Sodium Bromide.
                The data were collected at three different wavelength.</div>
              <div>I intend to use Autosol to solve this structure, but
                I am not sure which parameter I should put in the field:
                f' and f''?</div>
              <div>I think there is a table in the synchrotron but maybe
                I didn't notice about that. So now what should I do?</div>
              <div><br>
              </div>
              <div>Thank you very much for your input!</div>
              <div>Best wishes,<br clear="all">
                <br>
                -- <br>
                <b><span style="font-size: x-small;">
                    <div><span style="font-weight: normal; font-size:
                        small;"><b><span style="font-size: x-small;">TriNgo</span></b></span></div>
                  </span></b>
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                  border-left: 1px solid rgb(204, 204, 204);
                  padding-left: 1ex;"><span style="font-size: x-small;">Structural
                    Biology Lab</span></blockquote>
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                  <span style="font-size: x-small;">School of Medicine -
                    Sungkyunkwan University</span></blockquote>
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                  padding-left: 1ex;">
                  <span style="font-size: x-small;">Phone: 031-299-6150</span></blockquote>
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                    <div><br>
                      Thomas C. Terwilliger<br>
                      Mail Stop M888<br>
                      Los Alamos National Laboratory<br>
                      Los Alamos, NM 87545<br>
                      <br>
                      Tel:&nbsp; 505-667-0072&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; email: <a
                        moz-do-not-send="true"
                        href="mailto:terwilliger@LANL.gov">terwilliger@LANL.gov</a><br>
                      Fax: 505-665-3024&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; SOLVE web site:&nbsp;<a
                        moz-do-not-send="true"
                        href="http://solve.lanl.gov">http://solve.lanl.gov</a><br>
                      PHENIX web site: <a class="moz-txt-link-freetext" href="http:www.phenix-online.org">http:www.phenix-online.org</a><br>
                      ISFI Integrated Center for Structure and Function
                      Innovation web&nbsp;site:&nbsp;<a moz-do-not-send="true"
                        href="http://techcenter.mbi.ucla.edu">http://techcenter.mbi.ucla.edu</a><br>
                      TB Structural Genomics Consortium web site:&nbsp;<a
                        moz-do-not-send="true"
                        href="http://www.doe-mbi.ucla.edu/TB">http://www.doe-mbi.ucla.edu/TB</a></div>
                    <div>CBSS Center for Bio-Security Science web site:
                      <a moz-do-not-send="true"
                        href="http://www.lanl.gov/cbss">http://www.lanl.gov/cbss</a><br>
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