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<div><br></div><div><span class="Apple-style-span" style="font-family: Tahoma, Verdana, Arial, sans-serif; color: rgb(42, 42, 42); "><div style="line-height: 17px; ">Hi all,</div><div style="line-height: 17px; "><br style="line-height: 17px; "></div><div style="line-height: 17px; ">I have just finished doing molecular replacement and an initial round of refinement (with simulated annealing) on a number of different datasets. They have all be straightforward since most of them are either point mutants and/or co-crystal structures. However, two of the structures are giving me some trouble. The data appears to be of good quality and resolution (2.25 and 2.55�). The MR proceeds as normal with good scores and then in the subsequent round of refinement the maps (2Fo-Fc and Fo-Fc) look strange. The density is stretched or flattened in one direction. Can anyone help me with what I may be overlooking?</div><div style="line-height: 17px; "><br style="line-height: 17px; "></div><div style="line-height: 17px; ">Thanks in advance</div><div style="line-height: 17px; ">JW</div></span></div> </body>
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