<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN">
<html>
<head>
<meta content="text/html; charset=ISO-8859-1"
http-equiv="Content-Type">
</head>
<body bgcolor="#ffffff" text="#000000">
Hi Joel,<br>
<br>
it used to be the known issue for some rare cases until I fixed it a
few months ago. So:<br>
<br>
1) could you please try the latest PHENIX from nightly builds and
see if you still have this problem:<br>
<br>
<a class="moz-txt-link-freetext" href="http://www.phenix-online.org/download/nightly_builds.cgi">http://www.phenix-online.org/download/nightly_builds.cgi</a><br>
<br>
2) if you still have this problem even using the latest PHENIX, then
please send me the data and model (and, optionally the command you
used that lead to these maps) and I will investigate.<br>
<br>
Pavel.<br>
<br>
<br>
On 10/20/10 11:58 AM, Joel Weadge wrote:
<blockquote cite="mid:SNT110-W10DC61DCC6BD0FCF3C73F6D65C0@phx.gbl"
type="cite">
<style><!--
.hmmessage P
{
margin:0px;
padding:0px
}
body.hmmessage
{
font-size: 10pt;
font-family:Tahoma
}
--></style>
<div><br>
</div>
<div><span class="Apple-style-span" style="font-family:
Tahoma,Verdana,Arial,sans-serif; color: rgb(42, 42, 42);">
<div style="line-height: 17px;">Hi all,</div>
<div style="line-height: 17px;"><br style="line-height: 17px;">
</div>
<div style="line-height: 17px;">I have just finished doing
molecular replacement and an initial round of refinement
(with simulated annealing) on a number of different
datasets. They have all be straightforward since most of
them are either point mutants and/or co-crystal structures.
However, two of the structures are giving me some trouble.
The data appears to be of good quality and resolution (2.25
and 2.55Å). The MR proceeds as normal with good scores and
then in the subsequent round of refinement the maps (2Fo-Fc
and Fo-Fc) look strange. The density is stretched or
flattened in one direction. Can anyone help me with what I
may be overlooking?</div>
<div style="line-height: 17px;"><br style="line-height: 17px;">
</div>
<div style="line-height: 17px;">Thanks in advance</div>
<div style="line-height: 17px;">JW<br>
</div>
</span></div>
</blockquote>
<br>
</body>
</html>