<div>Hello Chris,</div>The standard question here is: Are you certain of your space group? That seems to be the main culprit behind R factors that wont decrease.<div>Can you provide some statistics showing your confidence in your space group?<br>
<div><br></div><div>Kelly Daughtry</div><div><br clear="all">*******************************************************<br>Kelly Daughtry, Ph.D.<br>Post-Doctoral Fellow, Raetz Lab<br>Biochemistry Department<br>Duke University<br>
Alex H. Sands, Jr. Building<br>303 Research Drive<br>RM 250<br>Durham, NC 27710<br>P: 919-684-5178<br>*******************************************************<br>
<br><br><div class="gmail_quote">On Wed, Jan 12, 2011 at 5:12 PM, Christopher W Davies <span dir="ltr"><<a href="mailto:cdavies@purdue.edu">cdavies@purdue.edu</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex;">
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I am having an issue with refining my structure. �I have a 2.36 A resolution dataset. �I have found a solution by molecular replacement in the space group P3221. �I have been refining the structure, however, my Rfactor/Rfree are not decreasing even though I have good density. �My current Rfactor/Rfree values are .40/.43. �Can anyone help me?<br>
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Thanks.<br>
<br>
Chris<br>
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