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<meta http-equiv="Content-Type" content="text/html; charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Yes, it was the remark portion of the pdb.<div><br></div><div>Thanks,</div><div><br><div>
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><div><div><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div><div>---------------------------------------------</div><div><span class="Apple-style-span">Nick Sennett </span></div><div>Wood Lab, Graduate Student</div><div>University of Georgia BMB</div><div>Davidson Life Sciences A428</div><div>(706) 583-0303</div><div><a href="mailto:nsennett@me.com">nsennett@gmail.com</a></div></div></div></span></span></span></span><br class="Apple-interchange-newline"><span></span></div></div></span><br class="Apple-interchange-newline"><span></span><span><img height="162" width="150" id="d252770c-d892-4627-b1b8-30049ca03536" apple-width="yes" apple-height="yes" src="cid:3DCE4F6D-E184-4DA2-A921-93D4311BE545"></span>
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<br><div><div>On Jan 18, 2011, at 3:00 PM, <<a href="mailto:phenixbb-request@phenix-online.org">phenixbb-request@phenix-online.org</a>></div><div> wrote:</div><br><blockquote type="cite"><div>I can't see any reason why this wouldn't work, but you're right, it's<br>running untwinned ML refinement despite the twin law being set<br>correctly. However, I just tried running the twinned example included<br>in Phenix (the porin-twin dataset, which can be set up as a tutorial<br>from the GUI), and it worked as expected. I'm downloading the latest<br>installer now to double-check, but I doubt it's much different. One<br>possibility is that the REMARK section in the input PDB file is giving<br>conflicting instructions. Could you please run this command and send<br>me the output (off-list, unless you're certain there isn't anything<br>secret in there)?<br><br>grep "REMARK" /Users/nick/k94e_208/Refine_30M/ligand_revised2-coot-0.pdb<br><br>thanks,<br>Nat<font class="Apple-style-span" color="#000000"><font class="Apple-style-span" color="#144FAE"><br></font></font></div></blockquote></div></div></body></html>