I would direct you to a conversation on the ccp4bb last year ( <a href="https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=CCP4BB;eb55b86e.1003">https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=CCP4BB;eb55b86e.1003</a> ). Don't ask me how I remember this but what you are looking for specifically is found in this reply by Artem Evdokimov.<br>
<br><pre><font style="font-family: arial,helvetica,sans-serif;" size="2">"</font><font style="font-family: arial,helvetica,sans-serif;" size="2">It's called cross-soaking and it is a fairly popular technique<br>especially in the industrial community where we sometimes have to work<br>
with hundreds of ligands.<br><br>I've done it on several occasions and mostly have been pretty happy<br>with the results. Having said this I also would like to point out that<br>soaking is not always the same as co-crystallization in terms of the<br>
ligand-protein interactions. An extreme case is shown in the paper<br>below (figure 5):<br><br><a href="http://www.iucr.org/cgi-bin/paper?sx5059" target="_blank">http://www.iucr.org/cgi-bin/paper?sx5059</a><br><br>PDBID: 2i4g, 2i4h<br>
<br>Cheers,<br><br>Artem</font><font style="font-family: arial,helvetica,sans-serif;" size="2">"</font><font face="arial,helvetica,sans-serif"><font size="2"><br><br>Hope this helps,<br><br>Katherine<br></font></font><br>
</pre><div class="gmail_quote">On Wed, Apr 6, 2011 at 11:21 AM, Yuri <span dir="ltr"><<a href="mailto:yuri.pompeu@ufl.edu">yuri.pompeu@ufl.edu</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin: 0pt 0pt 0pt 0.8ex; border-left: 1px solid rgb(204, 204, 204); padding-left: 1ex;">
Has anyone ever reported seeing 2 different binding confromations for a ligand that was soaked vs. the same ligand co-crystallized?<br><font color="#888888">
<br>
--<br>
Yuri Pompeu<br>
<br>
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