<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Hi, I have just added alpha1-3 and alpha1-6 fucoses to NAG. The Fucose in the monomer library has O1 in the alpha position, which is axial. However, after refinement, the fucose moves so it is linked beta in the equatorial position. I believe the alpha and beta linkages for Fucose may be reversed in Phenix. Please advise. <div><br></div><div>The links are below.<div><br></div><div><br></div><div><br></div><div><br></div><div><br></div><div><br></div><div> apply_cif_link {<br> data_link = "ALPHA1-3"<br> residue_selection_1 = "chain A and resname NAG and resid 3821"<br> residue_selection_2 = "chain A and resname FUC and resid 3824"<br> }<br> apply_cif_link {<br> data_link = "ALPHA1-6"<br> residue_selection_1 = "chain A and resname NAG and resid 3821"<br> residue_selection_2 = "chain A and resname FUC and resid 3823"<br><div> }<br></div></div></div><br><br><div>
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font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; -webkit-text-decorations-in-effect: none; text-indent: 0px; -webkit-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><span class="Apple-style-span" style="border-collapse: separate; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; color: rgb(0, 0, 0); font-family: Arial; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; -webkit-text-decorations-in-effect: none; text-indent: 0px; -webkit-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><span class="Apple-style-span" style="border-collapse: separate; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; color: rgb(0, 0, 0); font-family: Arial; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; -webkit-text-decorations-in-effect: none; text-indent: 0px; -webkit-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><span class="Apple-style-span" style="border-collapse: separate; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; color: rgb(0, 0, 0); font-family: Arial; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; -webkit-text-decorations-in-effect: none; text-indent: 0px; -webkit-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><div style="font-family: Arial; "><span class="Apple-style-span" style="font-family: Arial; "><span class="Apple-style-span" style="font-family: Arial; ">With my best regards,</span></span></div><div style="font-family: Arial; "><span class="Apple-style-span" style="font-family: Arial; "><span class="Apple-style-span" style="font-family: Arial; ">Tim</span></span></div><div style="font-family: Arial; "><br class="khtml-block-placeholder"></div><div style="font-family: Arial; "><span class="Apple-style-span" style="font-family: Arial; "><span class="Apple-style-span" style="font-family: Arial; ">Timothy A. Springer, Ph.D. <span class="Apple-converted-space"> </span><span class="Apple-converted-space"></span><span class="Apple-converted-space"></span></span></span><span class="Apple-converted-space"><span class="Apple-style-span" style="font-family: Arial; "></span></span><span class="Apple-converted-space"></span></div><div style="font-family: Arial; "><span class="Apple-style-span" style="font-family: Arial; "><span class="Apple-style-span" style="font-family: Arial; ">Latham Family Professor of Biological Chemistry and Molecular Pharmacology </span></span></div><div style="font-family: Arial; "><span class="Apple-style-span" style="font-family: Arial; ">Harvard Medical School <a href="http://idi.harvard.edu/springer">http://idi.harvard.edu/springer</a> </span></div><div style="font-family: Arial; "><span class="Apple-style-span" style="font-family: Arial; ">Immune Disease Institute <a href="mailto:springer@idi.harvard.edu">springer@idi.harvard.edu</a> </span></div><div style="font-family: Arial; "><span class="Apple-style-span" style="font-family: Arial; ">Program in Cellular and Molecular Medicine, Dept. Medicine</span></div><div style="font-family: Arial; "><span class="Apple-style-span" style="font-family: Arial; ">Div. Hematology Children's Hospital Boston</span></div><div style="font-family: Arial; "><span class="Apple-style-span" style="font-family: Arial; ">3 Blackfan Circle, Rm 3103 phone: 617-713-8200 </span></div><div style="font-family: Arial; "><span class="Apple-style-span" style="font-family: Arial; ">Boston MA 02115 fax: 617-713-8232 </span></div><div style="font-family: Arial; "><br class="khtml-block-placeholder"></div><br class="Apple-interchange-newline"></span></span></span></span></div></span></div></span><br class="Apple-interchange-newline"></div></span><br class="Apple-interchange-newline"></div></span><br class="Apple-interchange-newline"></div></span><br class="Apple-interchange-newline"></div></span><br class="Apple-interchange-newline"></div></span><br class="Apple-interchange-newline"></span><br class="Apple-interchange-newline">
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