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<br>Dear Jeff,<div><br></div><div>Thanks. Very helpful. </div><div><br></div><div>Regards,</div><div>Rajesh</div><div><br><div><div id="SkyDrivePlaceholder"></div><hr id="stopSpelling">Date: Fri, 9 Mar 2012 10:43:38 -0700<br>From: jjheadd@lbl.gov<br>To: phenixbb@phenix-online.org<br>Subject: Re: [phenixbb] NCS<br><br>Hi Rajesh,<BR>
On Mar 9, 2012 10:15 AM, "Rajesh kumar" <<a href="mailto:ccp4_sc@hotmail.com">ccp4_sc@hotmail.com</a>> wrote:<br>
><br>
> Dear All,<br>
><br>
> I used "find NCS restraints automatically", " NCS restraints", and NCS type-torsion angle. Log file has following lines suggesting that it has found NCS and has applied (Am I right?). [ PHENIX (phenix.refine: 1.7.3_928)]<br>
><br>
> Updating torsion NCS restraints...<br>
> Number of torsion NCS restraints: 4736<br>
><br>
> 1. PDB file header doesn't have anything written on NCS. I thought PDB file should have it. Am I missing something?<BR>
This is a great point. There should probably be NCS output in the PDB header. If you used torsion NCS restraints, a list of matching residues is printed in the .log file. <BR>
> 2. What is difference between NCS type, global and torsion angle, and when they are preferred over the other?<BR>
Global NCS uses Cartesian-based restraints, which force the matching chains to be identical. Torsion NCS restraints are new, and restrain matching torsion angles, but are flexible and allow differences between related chains.<BR>
In general torsion NCS restraints perform better, and are safe to use with even high resolution data where differences between chains can be more pronounced in the map. <BR>
If you have further questions please let me know.<BR>
Jeff<BR>
> Thanks<br>
> Rajesh<br>
><br>
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