Well they aren't conserved so I guess I'll have to do some manual remodelling. The new version of HHPred is nice about building you a quick homology model to start with though (using Modeller).<div><br></div><div>Thanks Tom!<br>
<br><div class="gmail_quote">On 22 March 2012 00:56, Terwilliger, Thomas C <span dir="ltr"><<a href="mailto:terwilliger@lanl.gov">terwilliger@lanl.gov</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
<div>
<div style="direction:ltr;font-size:10pt;font-family:Tahoma">Hi Morten,
<div><br>
</div>
<div>I asked about this...and if your input model has Cys-Cys disulfides and the sequence file also has these cysteine residues, then the disulfides should be maintained by Rosetta. If that isn't happening, let me know!</div>
<div><br>
</div>
<div>I hope that helps!</div>
<div>All the best,</div>
<div>Tom T</div>
<div><br>
<div style="font-size:16px;font-family:Times New Roman">
<hr>
<div style="direction:ltr"><font face="Tahoma" color="#000000"><b>From:</b> <a href="mailto:phenixbb-bounces@phenix-online.org" target="_blank">phenixbb-bounces@phenix-online.org</a> [<a href="mailto:phenixbb-bounces@phenix-online.org" target="_blank">phenixbb-bounces@phenix-online.org</a>] on behalf of Morten Groftehauge [<a href="mailto:mortengroftehauge.work@gmail.com" target="_blank">mortengroftehauge.work@gmail.com</a>]<br>
<b>Sent:</b> Wednesday, March 21, 2012 8:25 AM<br>
<b>To:</b> PHENIX user mailing list<br>
<b>Subject:</b> [phenixbb] Rosetta and disulphides<br>
</font><br>
</div><div><div class="h5">
<div></div>
<div>Hi phenix-bb'ers,
<div><br>
</div>
<div>Is there anyway to make phenix.mr_rosetta take disulphide bridges into account? It's a rather enormous restraint on conformational space after all (wait is that a constraint or restraint? I get confused).</div>
<div><br>
</div>
<div>Cheers,</div>
<div>Morten<br clear="all">
<div><br>
</div>
-- <br>
Morten K Gr�ftehauge, PhD�
<div>Pohl Group</div>
<div>Durham University</div>
<br>
</div>
</div>
</div></div></div>
</div>
</div>
</div>
<br>_______________________________________________<br>
phenixbb mailing list<br>
<a href="mailto:phenixbb@phenix-online.org">phenixbb@phenix-online.org</a><br>
<a href="http://phenix-online.org/mailman/listinfo/phenixbb" target="_blank">http://phenix-online.org/mailman/listinfo/phenixbb</a><br>
<br></blockquote></div><br><br clear="all"><div><br></div>-- <br>Morten K Gr�ftehauge, PhD�<div>Pohl Group</div><div>Durham University</div><br>
</div>