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Hi,<br>
<br>
<blockquote
cite="mid:1333711115.83050.YahooMailNeo@web121704.mail.ne1.yahoo.com"
type="cite">
<div style="color:#000; background-color:#fff; font-family:times
new roman, new york, times, serif;font-size:14pt"> <span>There
may be PDB files which shows different protonation state of
HIS, at least there is PDB file shows double protonated HIS.
At which resolution of the crystal<var id="yui-ie-cursor"></var>
can allow us to distinguish different protonation states of
HIS?</span></div>
</blockquote>
<br>
an example:<br>
<br>
M. Blakeley, F. Ruiz, R. Cachau, I. Hazemann, F. Meilleur, A.
Mitschler, S. Ginell, P. Afonine, O. Ventura, A. Cousido, M.
Haertlein, A. Joachimiak, D. Myles & A. Podjarny. Quantum model
of catalysis based on a mobile proton revealed by subatomic x-ray
and neutron diffraction studies of h-aldose reductase. (2008). PNAS.
105, 1844-1848.<br>
<br>
Pavel<br>
<br>
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