<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="font-size: 18px; ">Anyone willing to summarize Xtriage's algorithm that determines whether "<i>anisotropic induced noise amplification</i>" is present in diffraction data? What is this noise amplification? Is it anisotropic correction factors that over-fit the data thereby inducing noise?</span><div style="font-size: 18px; "><br></div><div style="font-size: 18px; ">Many thanks,</div><div><span class="Apple-style-span" style="font-size: 18px; ">Jim T</span><br style="font-size: 18px; "><div style="font-size: 18px; "><br></div><div style="font-size: 18px; "><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; ">---------------- Anisotropy analyses ----------------</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; min-height: 13px; font-size: 16px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; ">Anisotropy ( [MaxAnisoB-MinAnisoB]/[MaxAnisoB] ) : 3.596e-01</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; "> Anisotropic ratio p-value : 0.000e+00</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; min-height: 13px; font-size: 16px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; "> The p-value is a measure of the severity of anisotropy as observed in the PDB.</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; "> The p-value of 0.000e+00 indicates that roughly 100.0 % of datasets available in the PDB have</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; "> an anisotropy equal to or worse than this dataset.</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; min-height: 13px; font-size: 16px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; min-height: 13px; font-size: 16px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; ">For the resolution shell spanning between 2.36 - 2.20 Angstrom,</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; ">the mean I/sigI is equal to 3.37. 42.2 % of these intensities have</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; ">an I/sigI > 3. When sorting these intensities by their anisotropic</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; ">correction factor and analysing the I/sigI behavior for this ordered</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; ">list, we can gauge the presence of 'anisotropy induced noise amplification'</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; ">in reciprocal space.</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; min-height: 13px; font-size: 16px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; "> The quarter of Intensities *least* affected by the anisotropy correction show</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; "> <I/sigI> : 3.82e+00</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; "> Fraction of I/sigI > 3 : 5.01e-01 ( Z = 3.59 )</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; min-height: 13px; font-size: 16px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; "> The quarter of Intensities *most* affected by the anisotropy correction show</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; "> <I/sigI> : 2.02e+00</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 16px; "> Fraction of I/sigI > 3 : 1.84e-01 ( Z = 10.78 )</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; min-height: 13px; font-size: 16px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 18px; "><b>The combined Z-score of 11.36 indicates that there probably is significant</b></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 18px; "><b>systematic noise amplification that could possibly lead to artefacts in the</b></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Courier; font-size: 18px; "><b>maps or difficulties in refinement</b></div></div><div><br></div></div></body></html>