<html><body><div style="color:#000; background-color:#fff; font-family:arial, helvetica, sans-serif;font-size:10pt"><div>Hi all</div><div><br></div><div>I<span class="tab"> have omitted some region manually from the pdb and did annealing - cartesian using phenix.refinment, the resulting map has density (1.2 sigma) in 2fofc and also</span></div><div><span class="tab">seen 2.8 sigma negative density on the omitted region?</span></div><div><br></div><div><span class="tab">any suggestions?</span></div><div><br><span class="tab"></span></div><div><span class="tab">Rab<br></span></div><div><br></div><div></div></div></body></html>