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<div class="moz-cite-prefix">Hello Raj,<br>
<br>
do you have the link specified in you PDB file?<br>
Here is an example for an disulphide bond. Usually the link is the
line above the CRYST1 card.<br>
<br>
SSBOND 1 CYS A 123 CYS A 123
1555 8555 2.12 <br>
<br>
Best,<br>
Sabine<br>
<br>
<br>
<br>
<br>
<br>
On 02/12/2013 11:21 PM, Rajeshwer Sankhala wrote:<br>
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<p>Hello,</p>
<p>I can see additional density just next to Cys residues in my
protein structure and it looks like BME is covalently linked
to cys. I could form a disulfide bond between BME and Cys in
"Refmac 5 review restrain" but when i subject it for
refinement BME is thrown away (no disulfide bond). I also
given Cif file in refinement but it didnot help. I have a feel
that i am missing something in cif file. Can anyone suggest
step by step starting from adding BME to making a link with
BME (Cys-BME) and then refinement?</p>
<p> </p>
<p>Raj</p>
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