<html><head><meta http-equiv="Content-Type" content="text/html charset=iso-8859-1"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Thank you for the fast reply.<div>Were Ramachandran restraints in PHENIX reside now in the recent GUI's ? Once there were immediately visible as well as rotamer restraints.</div><div>FF <br><div apple-content-edited="true">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; ">Dr Felix Frolow <br>Professor of Structural Biology and Biotechnology, Department of Molecular Microbiology and Biotechnology<br>Tel Aviv University 69978, Israel<br><br>Acta Crystallographica F, co-editor<br><br>e-mail: <a href="mailto:mbfrolow@post.tau.ac.il">mbfrolow@post.tau.ac.il</a><br>Tel: ++972-3640-8723<br>Fax: ++972-3640-9407<br>Cellular: 0547 459 608</span>
</div>
<br><div><div>On Jul 23, 2013, at 18:25 , Pavel Afonine <<a href="mailto:PAfonine@lbl.gov">PAfonine@lbl.gov</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite">
<meta content="text/html; charset=ISO-8859-1" http-equiv="Content-Type">
<div bgcolor="#FFFFFF" text="#000000">
<div class="moz-cite-prefix">Hi Felix,<br>
<br>
</div>
<blockquote cite="mid:788D8854-FCA1-4E97-A972-7783BBA261F5@post.tau.ac.il" type="cite">
<meta http-equiv="Content-Type" content="text/html;
charset=ISO-8859-1">
<div>Hi Pavel</div>
According to your mail from yesterday I wonder how can I treat
Ramachandran outliers in very large molecules (close to 9000
residues). </blockquote>
<br>
- fix them manually first and then run refinement;<br>
- if after refinement some of them come back as outliers then you
consider them for including into Ramachandran restraints.<br>
<br>
Ramachandran restraints are good at keeping correct conformation
from becoming an outlier (due to poor density for instance), and NOT
for fixing wrong one.<br>
<br>
Pavel<br>
</div>
_______________________________________________<br>phenixbb mailing list<br><a href="mailto:phenixbb@phenix-online.org">phenixbb@phenix-online.org</a><br>http://phenix-online.org/mailman/listinfo/phenixbb<br></blockquote></div><br></div></body></html>