<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">That’s a reasonable explanation. Another possibility is that there really isn’t room in the asymmetric unit of your cubic unit cell for a 7kDa protein. That could either be because you’ve inadvertently crystallised something else, or because the true symmetry is lower (and therefore the asymmetric unit is larger).<div><br></div><div>The apparent symmetry could be higher than the true symmetry if you have a twinned crystal. What do the twinning tests say (particularly the intensity moment tests)?</div><div><br></div><div>Best wishes,</div><div><br></div><div>Randy Read</div><div><br><div><div>On 30 Apr 2014, at 00:17, Nathaniel Echols <<a href="mailto:nechols@lbl.gov">nechols@lbl.gov</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div dir="ltr">On Tue, Apr 29, 2014 at 5:34 PM, Subhani Bandara <span dir="ltr"><<a href="mailto:ramssb17@gmail.com" target="_blank">ramssb17@gmail.com</a>></span> wrote:<br><div class="gmail_extra"><div class="gmail_quote">
<blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div dir="ltr"><div>I am trying to solve the structure of 7 KDa protein with a cubic space group at 1.35A resolution with Phaser-MR. It gives the error massage saying "The composition you have entered has a protein/nucleic acid content of 300.3%, which is impossible. Decrease your composition" and stops. This appears even if I give one chain as search model and say there is only one component copy. How can I fix this.</div>
</div></blockquote><div><br></div><div>The most likely explanation is that your sequence file contains multiple copies - or perhaps it contains the entire sequence of a large protein and you're working with a single domain?</div>
<div><br></div><div>-Nat</div></div></div></div>
_______________________________________________<br>phenixbb mailing list<br><a href="mailto:phenixbb@phenix-online.org">phenixbb@phenix-online.org</a><br>http://phenix-online.org/mailman/listinfo/phenixbb<br></blockquote></div><br><div apple-content-edited="true">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div>------</div><div>Randy J. Read</div><div>Department of Haematology, University of Cambridge</div><div>Cambridge Institute for Medical Research Tel: + 44 1223 336500</div><div>Wellcome Trust/MRC Building Fax: + 44 1223 336827</div><div>Hills Road E-mail: <a href="mailto:rjr27@cam.ac.uk">rjr27@cam.ac.uk</a></div><div>Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk</div></span></span>
</div>
<br></div></body></html>