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<div style="direction: ltr;font-family: Tahoma;color: #000000;font-size: 10pt;">Hello,
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<div>Active site contains iron (FE2) coordinated by three histidines. </div>
<div>After Phaser the active site does not contain un-explained green density.</div>
<div>After Refinement blobs of various shapes appear in the vicinity and upto 3-4A of active site iron.</div>
<div>Refinement is done using occupancy and anomalous of iron options.</div>
<div>Buffers do not contain molecules that could fit in those blobs.</div>
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<div>Any suggestions?</div>
<div>Thank you</div>
<div>Egor</div>
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