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Well, this is embarrassing...the assign label window was hiding behind the graphics window.
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<div>Now that I do see the FEM, I have an observation about the FEM. Although the FEM is providing an informative assessment of the protein and bound ligands, the assessment of water is a little strange. There are several water molecules that have strong
2Fo-Fc density but absolutely zero FEM density. Even if I contour the FEM down to the lowest possible value (0.09 sigma in this case), I still see no FEM density on these waters. The waters in question have good hydrogen bonding to the protein and acceptable
B-factors (26-30 Ang^2). These are waters that I would normally keep in my final model. Has anyone else seen this behavior? </div>
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<div>Some examples are posted here:</div>
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<div><a href="http://faculty.missouri.edu/~tannerjj/FEMwaters/FEMwaters.html">http://faculty.missouri.edu/~tannerjj/FEMwaters/FEMwaters.html</a></div>
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<div>On Sep 7, 2014, at 2:51 PM, Pavel Afonine wrote:</div>
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<div bgcolor="#FFFFFF" text="#000000">Hi John,<br>
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it does not open via Auto Open MTZ.<br>
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start Coot then<br>
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File -> Open MTZ, mmCIF, fcf or phs -> the choose FEM,PHIFEM (there are two Fourier maps in this file : FEM and usual 2mFo-DFc).<br>
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Pavel<br>
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<div class="moz-cite-prefix">On 9/7/14 11:14 AM, Tanner, John J. wrote:<br>
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<blockquote cite="mid:B335D1E2-5277-4ED0-A4B9-AB1540CD08FA@missouri.edu" type="cite">
Has anyone had problems reading feature enhanced map mtz files into coot? I made an FEM on a linux computer with phenix.fem, which created an mtz file:
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<div>-rw-r--r-- 1 tannerjj tanner 10760960 Sep 7 12:21 fem.mtz</div>
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<div>As you can see, the mtz file is not empty. The fem job appeared to have ended normally, since the last line in the out file is</div>
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<div>FEM loop: done so far: 100%Time: 3112.3967</div>
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<div>However, when I read this mtz into coot, the map does not appear. There is no error message from coot. I tried autoinput mtz and read mtz. I'm using coot on a mac. I have not tested with coot on Linux.</div>
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<div>Thanks.</div>
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<div>Jack Tanner</div>
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John J. Tanner, PhD<br>
Professor of Biochemistry and Director of Graduate Admissions and Recruitment</div>
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Professor of Chemistry (Joint Appointment)</div>
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University of Missouri-Columbia<br>
125 Chemistry Building<br>
Columbia, MO 65211<br>
email: <a moz-do-not-send="true" href="mailto:tannerjj@missouri.edu">tannerjj@missouri.edu</a><br>
phone: 573-884-1280<br>
fax: 573-882-2754<br>
<a moz-do-not-send="true" href="http://faculty.missouri.edu/%7Etannerjj/tannergroup/tanner.html">http://faculty.missouri.edu/~tannerjj/tannergroup/tanner.html</a><br>
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