<html><body><div style="color:#000; background-color:#fff; font-family:HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif;font-size:12pt"><div id="yiv8941626429"><div><div style="color: rgb(0, 0, 0); background-color: rgb(255, 255, 255); font-family: HelveticaNeue, 'Helvetica Neue', Helvetica, Arial, 'Lucida Grande', sans-serif; font-size: 12pt;"><div id="yiv8941626429yui_3_16_0_6_1410186871429_10" class="yiv8941626429" style="">Dear all,</div><div id="yiv8941626429yui_3_16_0_6_1410186871429_10" class="yiv8941626429" style=""><br class="yiv8941626429" style=""></div><div id="yiv8941626429yui_3_16_0_6_1410186871429_10" style="background-color:transparent;" class="yiv8941626429">I am working on a 3.6A Se SAD data. Autosol followed by autobuild gave a decent map (as expected at this resolution) with a moderate trace. After building poly ala trace and assigning few resides in and around Se positions when I refine the model, all the
sharp features of the electron density map are lost. For example, a nice looking helical density becomes a tube. I presume that this happens because wilson B is high (~110 A2) and the map requires modification, sharpening etc. Is there a way to get the map with
similar sharp features after model building and refinement as auto build gives? How could I follow exactly the same steps of density modification etc which auto build does?</div><div id="yiv8941626429yui_3_16_0_6_1410186871429_10" style="background-color:transparent;" class="yiv8941626429"><br id="yiv8941626429yui_3_16_0_1_1410186871429_2821" class="yiv8941626429" style=""></div><div id="yiv8941626429yui_3_16_0_6_1410186871429_10" style="background-color:transparent;" class="yiv8941626429">Thanks,</div><div id="yiv8941626429yui_3_16_0_6_1410186871429_10" style="background-color:transparent;" class="yiv8941626429"><br id="yiv8941626429yui_3_16_0_1_1410186871429_2823" class="yiv8941626429" style=""></div><div id="yiv8941626429yui_3_16_0_6_1410186871429_10" style="background-color:transparent;" class="yiv8941626429">Ashu</div><div id="yiv8941626429yui_3_16_0_1_1410186871429_21006" class="yiv8941626429" style=""><br class="yiv8941626429"
id="yiv8941626429yui_3_16_0_8_1410186871429_5" style=""></div></div></div></div></div></body></html>