<html><body><div style="color:#000; background-color:#fff; font-family:HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif;font-size:12pt"><div id="yiv9543139437"><div><div style="color: rgb(0, 0, 0); background-color: rgb(255, 255, 255); font-family: HelveticaNeue, 'Helvetica Neue', Helvetica, Arial, 'Lucida Grande', sans-serif; font-size: 12pt;"><div id="yiv9543139437yui_3_16_0_6_1410186871429_10">Dear all,</div><div id="yiv9543139437yui_3_16_0_6_1410186871429_10"><br></div><div id="yiv9543139437yui_3_16_0_6_1410186871429_10" style="color: rgb(0, 0, 0); font-size: 16px; font-family: HelveticaNeue, 'Helvetica Neue', Helvetica, Arial, 'Lucida Grande', sans-serif; background-color: transparent; font-style: normal;">I am working on a 3.6A Se SAD data. Autosol followed by autobuild gave a decent map (as expected at this resolution) with a moderate trace. After building poly ala trace and assigning few resides in and around Se
positions when I refine the model, all the sharp features of the electron density map are lost. For example, a nice looking helical density becomes a tube. I presume that this happens because wilson B is high (~110 A2) and the map requires
modification, sharpening etc. Is there a way to get the map with similar sharp features after model building and refinement as auto build gives? How could I follow exactly the same steps of density modification etc which auto build does?</div><div id="yiv9543139437yui_3_16_0_6_1410186871429_10" style="color: rgb(0, 0, 0); font-size: 16px; font-family: HelveticaNeue, 'Helvetica Neue', Helvetica, Arial, 'Lucida Grande', sans-serif; background-color: transparent; font-style: normal;"><br id="yiv9543139437yui_3_16_0_1_1410186871429_2821"></div><div id="yiv9543139437yui_3_16_0_6_1410186871429_10" style="color: rgb(0, 0, 0); font-size: 16px; font-family: HelveticaNeue, 'Helvetica Neue', Helvetica, Arial, 'Lucida Grande', sans-serif; background-color: transparent; font-style: normal;">Thanks,</div><div id="yiv9543139437yui_3_16_0_6_1410186871429_10" style="color: rgb(0, 0, 0); font-size: 16px; font-family: HelveticaNeue, 'Helvetica Neue', Helvetica,
Arial, 'Lucida Grande', sans-serif; background-color: transparent; font-style: normal;"><br id="yiv9543139437yui_3_16_0_1_1410186871429_2823"></div><div id="yiv9543139437yui_3_16_0_6_1410186871429_10" style="color: rgb(0, 0, 0); font-size: 16px; font-family: HelveticaNeue, 'Helvetica Neue', Helvetica, Arial, 'Lucida Grande', sans-serif; background-color: transparent; font-style: normal;">Ashu</div></div></div></div></div></body></html>