<html><head><meta http-equiv="Content-Type" content="text/html; charset=UTF-8"></head><body ><div>Generate a different test set and repeat refinement. Do it twenty times. If R turns out to be systematically higher than Rfree even in few resolution shells, you have an interesting case worth looking at. If not, it's just noise.</div><div><br></div><div><br></div><div><div style="font-size:9px;color:#575757">Sent on a Sprint Samsung Galaxy S® III</div></div><br><br><div>-------- Original message --------</div><div>From: "Singh, Bishal" <b.singh@dkfz-heidelberg.de> </div><div>Date:11/20/2014 9:52 AM (GMT-05:00) </div><div>To: phenixbb@phenix-online.org </div><div>Subject: [phenixbb] Rwork > Rfree </div><div><br></div>Hello everybody,<br> While depositing a structure in PDB, I observed that Rwork was more than Rfree in the highest resolution shell and in a few more shells as well. I shall be delighted if somebody can suggest me the reasons and also what should I do now. <br><br>With regards,<br>Bishal<br>PhD student,<br>DKFZ, University of Heidelberg, Germany<br>_______________________________________________<br>phenixbb mailing list<br>phenixbb@phenix-online.org<br>http://phenix-online.org/mailman/listinfo/phenixbb<br></body>