<html><body><div style="color:#000; background-color:#fff; font-family:HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif;font-size:8px"><div id="yui_3_16_0_1_1421873316626_19750" class="" style="" dir="ltr"><font size="2" id="yui_3_16_0_1_1421873316626_20318">Dear ALL</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style="" dir="ltr"><font size="2"><br></font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style="" dir="ltr"><font size="2" id="yui_3_16_0_1_1421873316626_20054">when you start you refinment in phenix with ***.sca file, fraction of reflection in test set will be created. under <b>options </b>(bottom) in phenix refine, you could see that fraction of reflection in test set is 0.1 which means 10 %</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style="" dir="ltr"><font size="2" id="yui_3_16_0_1_1421873316626_20069">by looking to the PDB header for completely different structures (below) you will find different percentage but always FREE R VALUE TEST SET COUNT is around 3800. so it always take the same 3800 count regardless the number of reflections.</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style="" dir="ltr"><font size="2"><br></font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style="" dir="ltr"><font size="2" id="yui_3_16_0_1_1421873316626_20313">when you look in generated mtz file after the refinment, you see under R-free-flags(+) and R-free-flags(-) either 0 or 1. what does 0 or 1 mean?</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style="" dir="ltr"><font size="2"><br></font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style="" dir="ltr"><font size="2" id="yui_3_16_0_1_1421873316626_20317">thank you in advance for explaination.</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style="" dir="ltr"><font size="2"><br></font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style="" dir="ltr"><font size="2">TKDawoD</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style=""><br></font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_20010">----------------------------------------------------------------------------------------------------</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_20008">REMARK 3 NUMBER OF REFLECTIONS : 47312 </font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_20007">REMARK 3 NUMBER OF REFLECTIONS (NON-ANOMALOUS) : 24389 </font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_20006">REMARK 3 </font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_20005">REMARK 3 FIT TO DATA USED IN REFINEMENT.</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_20000">REMARK 3 R VALUE (WORKING + TEST SET) : 0.1673</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_20004">REMARK 3 R VALUE (WORKING SET) : 0.1636</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_20003">REMARK 3 FREE R VALUE : 0.2084</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_20002">REMARK 3 FREE R VALUE TEST SET SIZE (%) : 8.21 </font></div><div id="yui_3_16_0_1_1421873316626_19750" class=""><font size="2" class=""></font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_20001">REMARK 3 FREE R VALUE TEST SET COUNT : 3882</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_19952">------------------------------------------------------------------------------------------------------</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_19951">REMARK 3 NUMBER OF REFLECTIONS : 107005 </font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_19950">REMARK 3 NUMBER OF REFLECTIONS (NON-ANOMALOUS) : 56251 </font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_19949">REMARK 3 </font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_19948">REMARK 3 FIT TO DATA USED IN REFINEMENT.</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" id="yui_3_16_0_1_1421873316626_19925" class="" style="">REMARK 3 R VALUE (WORKING + TEST SET) : 0.1457</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_19947">REMARK 3 R VALUE (WORKING SET) : 0.15.34</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_19946">REMARK 3 FREE R VALUE : 0.1997</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_19945">REMARK 3 FREE R VALUE TEST SET SIZE (%) : 3.51 </font></div><div id="yui_3_16_0_1_1421873316626_19750" class=""><font size="2"></font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" class="" style="" id="yui_3_16_0_1_1421873316626_19944">REMARK 3 FREE R VALUE TEST SET COUNT : 3809</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" id="yui_3_16_0_1_1421873316626_19896">-----------------------------------------------------------------------------------------------------</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" id="yui_3_16_0_1_1421873316626_19895">NUMBER OF REFLECTIONS : 48150 </font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" id="yui_3_16_0_1_1421873316626_19894">REMARK 3 NUMBER OF REFLECTIONS (NON-ANOMALOUS) : 25296 </font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" id="yui_3_16_0_1_1421873316626_19897">REMARK 3 </font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" id="yui_3_16_0_1_1421873316626_19893">REMARK 3 FIT TO DATA USED IN REFINEMENT.</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" id="yui_3_16_0_1_1421873316626_19892">REMARK 3 R VALUE (WORKING + TEST SET) : 0.1507</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" id="yui_3_16_0_1_1421873316626_19891">REMARK 3 R VALUE (WORKING SET) : 0.1470</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" id="yui_3_16_0_1_1421873316626_19890">REMARK 3 FREE R VALUE : 0.1918</font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" id="yui_3_16_0_1_1421873316626_19889">REMARK 3 FREE R VALUE TEST SET SIZE (%) : 7.98 </font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><font size="2" id="yui_3_16_0_1_1421873316626_19888">REMARK 3 FREE R VALUE TEST SET COUNT : 3841 </font></div><div id="yui_3_16_0_1_1421873316626_19750" class="" style=""><br class="" style=""></div></div></body></html>