<html><body><div style="color:#000; background-color:#fff; font-family:HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, Sans-Serif;font-size:12px"><div dir="ltr" id="yui_3_16_0_1_1422717914778_13284"><br>Dear All,</div><div dir="ltr" id="yui_3_16_0_1_1422717914778_13294"> </div><div dir="ltr" id="yui_3_16_0_1_1422717914778_13295">After a Phenix refinement (for crystal 2.5A), my Rwork is about 0.19, and my Rfree is about 0.26. Is the gap between Rwork and Rfree too karge? If too large, why it is too large and how to avoid it?</div><div dir="ltr" id="yui_3_16_0_1_1422717914778_13316"> </div><div dir="ltr" id="yui_3_16_0_1_1422717914778_13315">I am looking forward to getting your reply.</div><div dir="ltr" id="yui_3_16_0_1_1422717914778_13314"> </div><div dir="ltr" id="yui_3_16_0_1_1422717914778_13321">Dialing</div></div></body></html>