<html><head> <meta http-equiv="Content-Type" content="text/html; charset=Windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; color: rgb(0, 0, 0); font-size: 14px; font-family: Calibri, sans-serif;"><div>What I meant is: I actually don’t have anomalous signal, so when I use I+/I-, kind of like I use Imean twice for each reflection. Can I do that?  My guess is that the resolution is low, so double reflection/parameter ratio really benefits the refinement, although overall the weight for x-ray term is not change, because rms bond and angle are similar.</div><div><br></div><div>Newer versions are better for my other refinement with higher resolution, but not this data set.</div><div><br></div><div>Guangyu</div><div><br></div><span id="OLK_SRC_BODY_SECTION"><div style="font-family:Calibri; font-size:11pt; text-align:left; color:black; BORDER-BOTTOM: medium none; BORDER-LEFT: medium none; PADDING-BOTTOM: 0in; PADDING-LEFT: 0in; PADDING-RIGHT: 0in; BORDER-TOP: #b5c4df 1pt solid; BORDER-RIGHT: medium none; PADDING-TOP: 3pt"><span style="font-weight:bold">From: </span> Pavel Afonine <<a href="mailto:pafonine@lbl.gov">pafonine@lbl.gov</a>><br><span style="font-weight:bold">Date: </span> Friday, February 6, 2015 at 2:12 PM<br><span style="font-weight:bold">To: </span> Guangyu Zhu <<a href="mailto:gzhu@hwi.buffalo.edu">gzhu@hwi.buffalo.edu</a>>, "<a href="mailto:phenixbb@phenix-online.org">phenixbb@phenix-online.org</a>" <<a href="mailto:phenixbb@phenix-online.org">phenixbb@phenix-online.org</a>><br><span style="font-weight:bold">Subject: </span> Re: [phenixbb] refinement with anomalous data<br></div><div><br></div><div><div bgcolor="#FFFFFF" text="#000000"><br><blockquote cite="mid:D0FA7A55.1219%25gzhu@hwi.buffalo.edu" type="cite"><div>I use same data set for refinement, so Rfree is also marked for same reflection. The only difference is that I chose “Data labels” from Phenix IMEAN or I(+) I(-). </div></blockquote><br> What I meant is that the IMEAN set is not the same as I(+),I(-) at least because the number of reflections in IMEAN is not the same as in I(+),I(-).<br><br><blockquote cite="mid:D0FA7A55.1219%25gzhu@hwi.buffalo.edu" type="cite"><div>By the way, I use version 1.8.2 for refinement. Newer versions gives higher R/Rfree. </div></blockquote><br> Should be the other way around...<br><br> Pavel<br><br></div></div></span></body></html>