<div dir="ltr"><div><div><div>Hi all,<br><br>As you might have noticed, there is a discussion going on about the fraudulent 2hr0 pdb deposition on the CCP4BB<br></div>Searching for 2hr0 I came across <a href="http://www.phenix-online.org/documentation/reference/cif_as_mtz.html">http://www.phenix-online.org/documentation/reference/cif_as_mtz.html</a> where 2hr0 is used as an example.<br><br></div>Might I suggest that this is changed in the documentation?<br><br></div>Best regards,<br>Folmer Fredslund<br><div><div><div><div><br><br><div><div class="gmail_quote">---------- Forwarded message ----------<br>From: <b class="gmail_sendername">Kerff Fred</b> <span dir="ltr"><<a href="mailto:fkerff@ulg.ac.be">fkerff@ulg.ac.be</a>></span><br>Date: 2015-02-06 11:58 GMT+01:00<br>Subject: [ccp4bb] Absence of contact between layers in a crystal<br>To: <a href="mailto:CCP4BB@jiscmail.ac.uk">CCP4BB@jiscmail.ac.uk</a><br><br><br>Hello,<br>
<br>
Looking at structure 2HR0 ("The structure of complement C3b provides insights into complement activation and regulation. »,Abdul Ajees, A., Gunasekaran, K., Volanakis, J.E., Narayana, S.V., Kotwal, G.J., Krishna Murthy, H.M.; (2006) Nature 444: 221-225), I noticed the absence of contacts between layers in the crystal. Is it something that has already been observed in other crystals?<br>
<br>
Best regards,<br>
<br>
Fred<br>
-----<br>
Frédéric Kerff<br>
Chercheur qualifié F.R.S.-FNRS<br>
Cristallographie des protéines<br>
Centre d'Ingénierie des Protéines<br>
Université de Liège<br>
17, Allée du 6 Août - Bat B5a<br>
4000 Liège (Belgium)<br>
Tel.: +32 (0)4 3663620<br>
Fax: +32 (0)4 3663772<br>
<br>
<br>
<br>
> Le 6 févr. 2015 à 10:12, Tim Gruene <<a href="mailto:tg@SHELX.UNI-AC.GWDG.DE">tg@SHELX.UNI-AC.GWDG.DE</a>> a écrit :<br>
><br>
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> Dear Smith,<br>
><br>
> The sca file most likely does not contain flags. pointless can read<br>
> the sca file, standardise it to ccp4 standards and freerflag marks<br>
> random reflections. You should use the maximum of 500 unique<br>
> reflections or 5% of the unique reflections, whichever is larger.<br>
><br>
> Best,<br>
> Tim<br>
><br>
> On 02/06/2015 09:49 AM, Smith Lee wrote:<br>
>> Dear All, I have a sca file. Will you please tell me by which<br>
>> software or how I can know whether the sca file contains R-free<br>
>> tags? If not, by which software or how I can add the R-free tags?<br>
>> And how much of the reflections I add the R-free tags? I am looking<br>
>> forward to getting your reply. Smith<br>
>><br>
><br>
> - --<br>
> - --<br>
> Dr Tim Gruene<br>
> Institut fuer anorganische Chemie<br>
> Tammannstr. 4<br>
> D-37077 Goettingen<br>
><br>
> GPG Key ID = A46BEE1A<br>
><br>
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> j0JFZac4kAh8twpov71MG84=<br>
> =XN57<br>
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</div><br><br clear="all"><br>-- <br><div class="gmail_signature">Folmer Fredslund<br></div>
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