<html><head></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; color: rgb(0, 0, 0); font-size: 14px; font-family: Calibri, sans-serif; "><div>Hello everyone,</div><div> I am refining a structure at 2.3 A resolution. R-work/R-free is 26/31 and is not reducing further. When I looked into the resolution bin, I notices a sharp rise of R-free between resolution range 2.5-2.6 A (marked in figure below). However R-work is fine. I am doubtful of reducing the resolution to 2.5 A or lower. Please suggest me your opinion. Your words will be highly appreciated.</div><div><br></div><div><img src="cid:D50E01B9-02E4-422D-BDA2-47C533F98358" type="image/png"></div><div><br></div><div>With regards,</div><div>Bishal Singh</div><div>PhD Student</div><div>University of Heidelberg, Germany</div></body></html>